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Home / ELISA Kit

Human Tyrosine-protein phosphatase non-receptor type 22 (PTPN22) ELISA Kit

SKU : BHE13703267

Protein tyrosine phosphatase, non-receptor type 22 (lymphoid) is located on the short arm of Chromosome 1 near the telomere (1p13.2) on the Crick (minus) strand. It is 57,898 bases in length and encodes a protein of 807 amino acids (molecular weight 91,705 Da). There are 24 exons in the gene and 21 transcript variants encoding 10 distinct proteins are known. The proteins are located in the cytoplasm.
This gene encodes a protein tyrosine phosphatase which is expressed primarily in lymphoid tissues. This enzyme associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T cell receptor signaling pathway.

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Mfr.No.	KTE61051
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Tyrosine-protein phosphatase non-receptor type 22 (PTPN22) ELISA Kit employs a two-site sandwich ELISA to quantitate PTPN22.
Alternative Names	PTPN22; LYP; LYP1; LYP2; PEP; PTPN8; PEST-domain phosphatase; lymphoid-specific protein tyrosine phosphatase; protein tyrosine phosphatase; non-receptor type 8
Detection Method	Colorimetric
Kit Components	Human Tyrosine-protein phosphatase non-receptor type 22 microplate Human Tyrosine-protein phosphatase non-receptor type 22 standard Human Tyrosine-protein phosphatase non-receptor type 22 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Tyrosine-protein phosphatase non-receptor type 22 (PTPN22) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTPN22. No significant cross-reactivity or interference between Human PTPN22 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Protein tyrosine phosphatase, non-receptor type 22 (lymphoid) is located on the short arm of Chromosome 1 near the telomere (1p13.2) on the Crick (minus) strand. It is 57,898 bases in length and encodes a protein of 807 amino acids (molecular weight 91,705 Da). There are 24 exons in the gene and 21 transcript variants encoding 10 distinct proteins are known. The proteins are located in the cytoplasm. This gene encodes a protein tyrosine phosphatase which is expressed primarily in lymphoid tissues. This enzyme associates with the molecular adapter protein CBL and may be involved in regulating CBL function in the T cell receptor signaling pathway.
Notes	This Human Tyrosine-protein phosphatase non-receptor type 22 (PTPN22) ELISA Kit employs a two-site sandwich ELISA to quantitate PTPN22 in samples. An antibody specific for PTPN22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTPN22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPN22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPN22 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	26191

Applications	ELISA
Applications Notes	This Human Tyrosine-protein phosphatase non-receptor type 22 (PTPN22) ELISA Kit employs a two-site sandwich ELISA to quantitate PTPN22 in samples. An antibody specific for PTPN22 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTPN22 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPN22 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPN22 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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