Human Tyrosine Protein Phosphatase Non-receptor Type 11

Mfr.No.

E2445Hu

Alternative Names

BPTP3;CFC;JMML;NS1;Protein-tyrosine phosphatase 1D;Protein-tyrosine phosphatase 2C;PTP-1D;PTP2C;PTP-2C;PTPN11;SHP2;SHP-2;SH-PTP2;SH-PTP3;Tyrosine-protein phosphatase non-receptor type 11

Reactivity

Human

Detection Range

20-3800ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

68011

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.61ng/L

Background

Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus (PubMed:10655584, PubMed:18559669, PubMed:18829466, PubMed:26742426, PubMed:28074573). Positively regulates MAPK signal transduction pathway (PubMed:28074573). Dephosphorylates GAB1, ARHGAP35 and EGFR (PubMed:28074573). Dephosphorylates ROCK2 at 'Tyr-722' resulting in stimulation of its RhoA binding activity (PubMed:18559669). Dephosphorylates CDC73 (PubMed:26742426). Dephosphorylates SOX9 on tyrosine residues, leading to inactivate SOX9 and promote ossification (By similarity).

Gene ID	5781
UniProt ID	Q06124
Antigen	PTPN11

Research Area

Others

Protocol

These standard curves of E2445Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	2.174	2.278	2.251
	2.382
1000ng/L	1.389	1.334	1.307
	1.28
500ng/L	0.579	0.592	0.565
	0.606
250ng/L	0.416	0.431	0.404
	0.446
125ng/L	0.209	0.198	0.17
	0.186
0ng/L	0.028	0.027	0
	0.027

Precision

We measured random samples of E2445Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	411.47	18.65	4.53
Intra-Assay	2	18	538.45	24.35	4.52
Intra-Assay	3	18	1509.95	44.08	2.92

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103983	E2445Hu	48T	$320		Add to Cart
BHE12103983	E2445Hu	96T	$458		Add to Cart

Human Tyrosine Protein Phosphatase Non-receptor Type 11 - PTPN11 ELISA Kit

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