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Home / ELISA Kit

Human Tyrosine-protein kinase Lyn (LYN) ELISA Kit

SKU : BHE13703979

Using a v-yes DNA as the probe, Yamanashi et al. (1987) screened a human cDNA library made from placental RNA and derived DNA clones representing a novel genetic locus termed LYN. Nucleotide sequencing showed that LYN encodes a novel tyrosine kinase. Northern hybridization analysis showed that a 3.2-kb LYN mRNA was expressed in a variety of tissues of the human fetus. The pattern of expression was different from those of related genes such as YES.The degranulation response was dependent on a rise in intracellular calcium that was inhibited in Lyn-deficient mast cells but intact in Fyn-deficient cells. Degranulation proceeded in Lyn -/- cells due to increased activation and constitutive phosphorylation of the calcium-independent protein kinase C delta isoform (PRKCD).

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Mfr.No.	KTE61764
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Tyrosine-protein kinase Lyn (LYN) ELISA Kit employs a two-site sandwich ELISA to quantitate LYN.
Alternative Names	LYN; FLJ26625; JTK8; Yamaguchi sarcoma viral (v-yes-1) oncogene homolog; Yamaguchi sarcoma viral (v-yes-1) related oncogene homolog
Detection Method	Colorimetric
Kit Components	Human Tyrosine-protein kinase Lyn microplate Human Tyrosine-protein kinase Lyn standard Human Tyrosine-protein kinase Lyn detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Tyrosine-protein kinase Lyn (LYN) ELISA Kit has high sensitivity and excellent specificity for detection of Human LYN. No significant cross-reactivity or interference between Human LYN and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Using a v-yes DNA as the probe, Yamanashi et al. (1987) screened a human cDNA library made from placental RNA and derived DNA clones representing a novel genetic locus termed LYN. Nucleotide sequencing showed that LYN encodes a novel tyrosine kinase. Northern hybridization analysis showed that a 3.2-kb LYN mRNA was expressed in a variety of tissues of the human fetus. The pattern of expression was different from those of related genes such as YES.The degranulation response was dependent on a rise in intracellular calcium that was inhibited in Lyn-deficient mast cells but intact in Fyn-deficient cells. Degranulation proceeded in Lyn -/- cells due to increased activation and constitutive phosphorylation of the calcium-independent protein kinase C delta isoform (PRKCD).
Notes	This Human Tyrosine-protein kinase Lyn (LYN) ELISA Kit employs a two-site sandwich ELISA to quantitate LYN in samples. An antibody specific for LYN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLYN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LYN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LYN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4067

Applications	ELISA
Applications Notes	This Human Tyrosine-protein kinase Lyn (LYN) ELISA Kit employs a two-site sandwich ELISA to quantitate LYN in samples. An antibody specific for LYN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLYN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LYN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LYN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
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