Human Tyrosine-protein kinase ABL1(ABL1) ELISA kit

Human Tyrosine-protein kinase ABL1(ABL1) ELISA kit

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Mfr.No.

CSB-EL001105HU

Sensitivity

6.25 pg/mL

Detection Range

25 pg/mL-1600 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

c-abl oncogene 1, receptor tyrosine kinase, RP11-83J21.1, ABL, JTK7, bcr/abl, c-ABL, p150, v-abl, bcr/c-abl oncogene protein, proto-oncogene tyrosine-protein kinase ABL1, v-abl Abelson murine leukemia viral oncogene homolog 1

Recovery

The recovery of human PPIA/CYPA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-94
EDTA plasma (n=4)	95	89-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human PPIA/CYPA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-95
1:2	Average %	100
	Range %	95-104
1:4	Average %	90
	Range %	85-94
1:8	Average %	98
	Range %	92-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
200	2.079	2.035	2.057	1.948
100	1.594	1.576	1.585	1.476
50	0.849	0.831	0.840	0.731
25	0.446	0.439	0.443	0.334
12.5	0.299	0.284	0.292	0.183
6.25	0.204	0.198	0.201	0.092
3.12	0.153	0.158	0.156	0.047
0	0.110	0.108	0.109

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P00519

Applications	ELISA
Research Area	ABL1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504754	CSB-EL001105HU	96T	$695	Add to Cart
BHE10504754	CSB-EL001105HU	5x96T	$3336	Add to Cart
BHE10504754	CSB-EL001105HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.