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Home / ELISA Kit

Human TYRO protein tyrosine kinase-binding protein (TYROBP) ELISA Kit

SKU : BHE13702327

TYROBP encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL.

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Mfr.No.	KTE60108
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human TYRO protein tyrosine kinase-binding protein (TYROBP) ELISA Kit employs a two-site sandwich ELISA to quantitate TYROBP.
Alternative Names	TYROBP; DAP12; KARAP; PLOSL; DNAX-activation protein 12; killer activating receptor associated protein
Detection Method	Colorimetric
Kit Components	Human TYRO protein tyrosine kinase-binding protein microplate Human TYRO protein tyrosine kinase-binding protein standard Human TYRO protein tyrosine kinase-binding protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human TYRO protein tyrosine kinase-binding protein (TYROBP) ELISA Kit has high sensitivity and excellent specificity for detection of Human TYROBP. No significant cross-reactivity or interference between Human TYROBP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TYROBP encodes a transmembrane signaling polypeptide which contains an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. The encoded protein may associate with the killer-cell inhibitory receptor (KIR) family of membrane glycoproteins and may act as an activating signal transduction element. This protein may bind zeta-chain (TCR) associated protein kinase 70kDa (ZAP-70) and spleen tyrosine kinase (SYK) and play a role in signal transduction, bone modeling, brain myelination, and inflammation. Mutations within this gene have been associated with polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL), also known as Nasu-Hakola disease. Its putative receptor, triggering receptor expressed on myeloid cells 2 (TREM2), also causes PLOSL.
Notes	This Human TYRO protein tyrosine kinase-binding protein (TYROBP) ELISA Kit employs a two-site sandwich ELISA to quantitate TYROBP in samples. An antibody specific for TYROBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTYROBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TYROBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TYROBP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	7305

Applications	ELISA
Applications Notes	This Human TYRO protein tyrosine kinase-binding protein (TYROBP) ELISA Kit employs a two-site sandwich ELISA to quantitate TYROBP in samples. An antibody specific for TYROBP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTYROBP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TYROBP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TYROBP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702327	KTE60108	48 T	$339		Add to Cart
BHE13702327	KTE60108	96 T	$589		Add to Cart
BHE13702327	KTE60108	96 Tx5	$2629		Add to Cart
BHE13702327	KTE60108	96 Tx50	$24569		Add to Cart

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