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Home / ELISA Kit

Human Twinfilin-1 (TWF1) ELISA Kit

SKU : BHE13702334

TWF1 encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1.
The deduced 350-amino acid protein has a calculated molecular mass of 40.3 kD. TWF1 has an N-myristoylation site and several sites for threonine, serine, and tyrosine phosphorylation, but it lacks a conserved protein kinase catalytic domain. Northern blot analysis detected a 3.4-kb transcript that was highly expressed in colon, testis, uterus, ovary, prostate, and lung. Lower expression was detected in brain, bladder, and heart, and no expression was detected in liver.

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Mfr.No.	KTE60115
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Twinfilin-1 (TWF1) ELISA Kit employs a two-site sandwich ELISA to quantitate TWF1.
Alternative Names	TWF1; A6; MGC23788; MGC41876; PTK9; A6 protein tyrosine kinase; PTK9 protein tyrosine kinase 9; protein tyrosine kinase 9; twinfilin 1
Detection Method	Colorimetric
Kit Components	Human Twinfilin-1 microplate Human Twinfilin-1 standard Human Twinfilin-1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Twinfilin-1 (TWF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TWF1. No significant cross-reactivity or interference between Human TWF1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TWF1 encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. The deduced 350-amino acid protein has a calculated molecular mass of 40.3 kD. TWF1 has an N-myristoylation site and several sites for threonine, serine, and tyrosine phosphorylation, but it lacks a conserved protein kinase catalytic domain. Northe blot analysis detected a 3.4-kb transcript that was highly expressed in colon, testis, uterus, ovary, prostate, and lung. Lower expression was detected in brain, bladder, and heart, and no expression was detected in liver.
Notes	This Human Twinfilin-1 (TWF1) ELISA Kit employs a two-site sandwich ELISA to quantitate TWF1 in samples. An antibody specific for TWF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTWF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TWF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TWF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	5756

Applications	ELISA
Applications Notes	This Human Twinfilin-1 (TWF1) ELISA Kit employs a two-site sandwich ELISA to quantitate TWF1 in samples. An antibody specific for TWF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTWF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TWF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TWF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
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