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Home / ELISA Kit

Human Tumor suppressor p53-binding protein 1 (TP53BP1) ELISA Kit

SKU : BHE13702374

TP53BP1 has a predicted molecular mass of 217 kD and shows homology in its C-terminal 247 amino acids to the C terminus of the tumor suppressor protein BRCA1. TP53BP1 was present in both the cytoplasm and nucleus in some cells and only in the nucleus in others. Furthermore, there were 2 nuclear staining patterns, one homogeneous staining and the other dot staining.TP53BP1 was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation. TP53BP1 played a partially redundant role in phosphorylation of the downstream checkpoint effector proteins BRCA1 and CHK2 but was required for the formation of BRCA1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage.

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Mfr.No.	KTE60155
Description	This Human Tumor suppressor p53-binding protein 1 (TP53BP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TP53BP1.
Alternative Names	TP53BP1; 53BP1; FLJ41424; MGC138366; p202; tumor protein 53-binding protein; 1; tumor protein p53-binding protein; 1
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Tumor suppressor p53-binding protein 1 microplate Human Tumor suppressor p53-binding protein 1 standard Human Tumor suppressor p53-binding protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Tumor suppressor p53-binding protein 1 (TP53BP1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TP53BP1. No significant cross-reactivity or interference between Human TP53BP1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TP53BP1 has a predicted molecular mass of 217 kD and shows homology in its C-terminal 247 amino acids to the C terminus of the tumor suppressor protein BRCA1. TP53BP1 was present in both the cytoplasm and nucleus in some cells and only in the nucleus in others. Furthermore, there were 2 nuclear staining patterns, one homogeneous staining and the other dot staining.TP53BP1 was required for p53 accumulation, G2/M checkpoint arrest, and the intra-S-phase checkpoint in response to ionizing radiation. TP53BP1 played a partially redundant role in phosphorylation of the downstream checkpoint effector proteins BRCA1 and CHK2 but was required for the formation of BRCA1 foci in a hierarchical branched pathway for the recruitment of repair and signaling proteins to sites of DNA damage.
Notes	This Human Tumor suppressor p53-binding protein 1 (TP53BP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TP53BP1 in samples. An antibody specific for TP53BP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTP53BP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TP53BP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TP53BP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	7158
Antigen	TP53BP1
Tag	TP53BP1
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Tumor suppressor p53-binding protein 1 (TP53BP1) ELISA Kit employs a two-site sandwich ELISA to quantitate TP53BP1 in samples. An antibody specific for TP53BP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTP53BP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TP53BP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TP53BP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702374	KTE60155	48 T	$339		Add to Cart
BHE13702374	KTE60155	96 T	$589		Add to Cart
BHE13702374	KTE60155	96 Tx5	$2629		Add to Cart
BHE13702374	KTE60155	96 Tx50	$24569		Add to Cart

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