Human tumor necrosis factor-related apoptosis-inducing ligand receptor 3,TRAIL-R3 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E04748h

Sensitivity

19.5 pg/mL

Detection Range

78 pg/mL-5000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Alternative Names

tumor necrosis factor-related apoptosis-inducing ligand 3,TRAIL-R3

Recovery

The recovery of Human WNT5B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-102
EDTA plasma (n=4)	85	80-90

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of Human WNT5B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	98
	Range %	94-102
1:4	Average %	94
	Range %	89-98
1:8	Average %	85
	Range %	80-89

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O14798

Applications	ELISA
Research Area	TRAIL-R3

Storage	Store at 2-8°C. Please refer to protocol.

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