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Home / ELISA Kit

Human Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Receptor 2 (TRAIL-R2/DR5) ELISA Kit

SKU : BHE13702445

Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Receptor 2 (TRAIL-R2/DR5) is a member of the TNF-receptor superfamily, and contains an intracellular death domain. TRAIL-R2/DR5 can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by TRAIL-R2/DR5. Two transcript variants encoding different isoforms and one non-coding transcript have been found for TRAIL-R2/DR5.

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Mfr.No.	KTE60227
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Receptor 2 (TRAIL-R2/DR5) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF10B.
Alternative Names	TNFRSF10B; CD262; DR5; KILLER; KILLER/DR5; TRAIL-R2; TRAILR2; TRICK2; TRICK2A; TRICK2B; TRICKB; ZTNFR9; Fas-like protein; TNF-related apoptosis-inducing ligand receptor 2; apoptosis inducing protein TRICK2A/2B; a
Detection Method	Colorimetric
Kit Components	Human Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Receptor 2 microplate Human Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Receptor 2 standard Human Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Receptor 2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Receptor 2 (TRAIL-R2/DR5) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFRSF10B. No significant cross-reactivity or interference between Human TNFRSF10B and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Receptor 2 (TRAIL-R2/DR5) is a member of the TNF-receptor superfamily, and contains an intracellular death domain. TRAIL-R2/DR5 can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by TRAIL-R2/DR5. Two transcript variants encoding different isoforms and one non-coding transcript have been found for TRAIL-R2/DR5.
Notes	This Human Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Receptor 2 (TRAIL-R2/DR5) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF10B in samples. An antibody specific for TNFRSF10B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF10B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF10B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF10B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	8795

Applications	ELISA
Applications Notes	This Human Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Receptor 2 (TRAIL-R2/DR5) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF10B in samples. An antibody specific for TNFRSF10B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF10B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF10B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF10B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702445	KTE60227	48 T	$399		Add to Cart
BHE13702445	KTE60227	96 T	$739		Add to Cart
BHE13702445	KTE60227	96 Tx5	$3339		Add to Cart
BHE13702445	KTE60227	96 Tx50	$29639		Add to Cart

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