Human Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Receptor 2(TRAIL-R2/DR5) ELISA Kit

Human Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Receptor 2(TRAIL-R2/DR5) ELISA Kit

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Mfr.No.

CSB-E13164h

Sensitivity

7.8 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

tumor necrosis factor receptor superfamily, member 10b, CD262, DR5, KILLER, KILLER/DR5, TRAIL-R2, TRAILR2, TRICK2, TRICK2A, TRICK2B, TRICKB, ZTNFR9, Fas-like protein, TNF-related apoptosis-inducing ligand receptor 2, apoptosis inducing protein TRICK2A/2B, a

Recovery

The recovery of bovine Cu/Zn-SOD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	101	103-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine Cu/Zn-SOD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-104
1:2	Average %	91
	Range %	85-97
1:4	Average %	90
	Range %	88-95
1:8	Average %	100
	Range %	96-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
1000	2.014	2.115	2.065	1.929
500	1.537	1.640	1.589	1.453
250	1.201	1.108	1.155	1.019
125	0.878	0.844	0.861	0.725
62.5	0.541	0.555	0.548	0.412
31.2	0.342	0.329	0.336	0.200
15.6	0.251	0.272	0.262	0.126
0	0.137	0.135	0.136

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O14763

Applications	ELISA
Research Area	TNFRSF10B

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10504734	CSB-E13164h	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.