Human Tumor Necrosis Factor Receptor Superfamily Member 5

Mfr.No.

E3153Hu

Description

This sandwich kit is for the accurate quantitative detection of Human Thymidine Kinase 1, Soluble (also known as TK1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

B-cell surface antigen CD40;Bp50;CD 40;CD antigen CD40;CD40;CD40L receptor;CDw40;Tumor necrosis factor receptor superfamily member 5

Reactivity

Human

Detection Range

0.5-200ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

30619

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.25ng/ml

Background

Receptor for TNFSF5/CD40LG (PubMed: 31331973). Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion (By similarity).

Gene ID	958
UniProt ID	P25942
Antigen	CD40

Research Area

Immunology; Signal Transduction

Protocol

These standard curves of E3153Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	2.049	2.059	2.013
	2.069
60ng/ml	1.751	1.656	1.611
	1.561
30ng/ml	1.021	1.055	1.009
	1.088
15ng/ml	0.706	0.7	0.655
	0.694
7.5ng/ml	0.423	0.41	0.365
	0.397
0ng/ml	0.059	0.045	0
	0.032

Precision

We measured random samples of E3153Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	34.3	2.25	6.6
Intra-Assay	2	18	38.3	1.82	4.7
Intra-Assay	3	18	13.2	0.86	6.5

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104684	E3153Hu	48T	$320		Add to Cart
BHE12104684	E3153Hu	96T	$458		Add to Cart

Human Tumor Necrosis Factor Receptor Superfamily Member 5 - TNFRSF5 ELISA Kit

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