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Home / ELISA Kit

Human Tumor necrosis factor receptor superfamily member 14 (TNFRSF14) ELISA Kit

SKU : BHE13702438

Tumor necrosis factor receptor superfamily member 14 is a member of the TNF-receptor superfamily. This receptor was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of the viral entry mechanism.
The cytoplasmic region of this receptor was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response.In melanocytic cells TNFRSF14 gene expression may be regulated by MITF.Widely expressed, with the highest expression in lung, spleen and thymus.

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Mfr.No.	KTE60220
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Tumor necrosis factor receptor superfamily member 14 (TNFRSF14) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF14.
Alternative Names	TNFRSF14; RP3-395M20.6; ATAR; HVEA; HVEM; LIGHTR; TR2; CD40-like protein; herpesvirus entry mediator A; tumor necrosis factor receptor superfamily; member 14; tumor necrosis factor receptor-like gene2
Detection Method	Colorimetric
Kit Components	Human Tumor necrosis factor receptor superfamily member 14 microplate Human Tumor necrosis factor receptor superfamily member 14 standard Human Tumor necrosis factor receptor superfamily member 14 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Tumor necrosis factor receptor superfamily member 14 (TNFRSF14) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFRSF14. No significant cross-reactivity or interference between Human TNFRSF14 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Tumor necrosis factor receptor superfamily member 14 is a member of the TNF-receptor superfamily. This receptor was identified as a cellular mediator of herpes simplex virus (HSV) entry. Binding of HSV viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of the viral entry mechanism. The cytoplasmic region of this receptor was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response.In melanocytic cells TNFRSF14 gene expression may be regulated by MITF.Widely expressed, with the highest expression in lung, spleen and thymus.
Notes	This Human Tumor necrosis factor receptor superfamily member 14 (TNFRSF14) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF14 in samples. An antibody specific for TNFRSF14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	8764

Applications	ELISA
Applications Notes	This Human Tumor necrosis factor receptor superfamily member 14 (TNFRSF14) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFRSF14 in samples. An antibody specific for TNFRSF14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFRSF14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFRSF14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFRSF14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702438	KTE60220	48 T	$339		Add to Cart
BHE13702438	KTE60220	96 T	$589		Add to Cart
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BHE13702438	KTE60220	96 Tx50	$24569		Add to Cart

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