Human Tumor Necrosis Factor Ligand Superfamily Member 10

Mfr.No.

E3154Hu

Description

This sandwich kit is for the accurate quantitative detection of human Tumor Necrosis Factor Ligand Superfamily Member 10 (also known as Tnfsf10/Trail) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Apo-2 ligand;Apo-2L;CD antigen CD253;Protein TRAIL;TNF-related apoptosis-inducing ligand;TNFSF 10;TNFSF10;TRAIL;Tumor necrosis factor ligand superfamily member 10;

Reactivity

Human

Detection Range

5-1500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

32509

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.49ng/L

Background

Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG (PubMed: 26457518, PubMed: 10549288). Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.

Gene ID	8743
UniProt ID	P50591
Antigen	TNFSF10

Research Area

Cancer

Protocol

These standard curves of E3154Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.386	2.528	2.469
	2.669
400ng/L	1.455	1.512	1.453
	1.568
200ng/L	0.732	0.76	0.701
	0.787
100ng/L	0.4	0.397	0.338
	0.394
50ng/L	0.255	0.257	0.198
	0.26
0ng/L	0.062	0.059	0
	0.056

Precision

We measured random samples of E3154Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	296.1	17.35	5.9
Intra-Assay	2	18	373	11.96	3.2
Intra-Assay	3	18	117	6.06	5.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104685	E3154Hu	48T	$320		Add to Cart
BHE12104685	E3154Hu	96T	$458		Add to Cart

Human Tumor Necrosis Factor Ligand Superfamily Member 10 - TNFSF10 ELISA Kit

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