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Home / ELISA Kit

Human Tumor necrosis factor-inducible gene 6 protein (TNFAIP6) ELISA Kit

SKU : BHE13702451

TSG-6 is a 30 kDa secreted protein that contains a hyaluronan-binding LINK domain a and thus is a member of the hyaluronan-binding protein family, also called hyaladherins. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable, covalent complex with inter-alpha-inhibitor (IalphaI), and thus enhance the serine protease inhibitory activity of IalphaI, which is important in the protease network associated with inflammation. The expression of this gene can be induced by a number of signalling molecules, principally tumor necrosis factor alpha (TNF-alpha) and interleukin-1 (IL-1). The expression can also be induced by mechanical stimuli in vascular smooth muscle cells, and is found to be correlated with proteoglycan synthesis and aggregation.

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Mfr.No.	KTE60233
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Tumor necrosis factor-inducible gene 6 protein (TNFAIP6) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFAIP6.
Alternative Names	TNFAIP6; TSG-6; TSG6; hyaluronate-binding protein; tumor necrosis factor alpha-inducible protein 6; tumor necrosis factor-inducible protein 6; tumor necrosis factor-stimulated gene-6 protein
Detection Method	Colorimetric
Kit Components	Human Tumor necrosis factor-inducible gene 6 protein microplate Human Tumor necrosis factor-inducible gene 6 protein standard Human Tumor necrosis factor-inducible gene 6 protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Tumor necrosis factor-inducible gene 6 protein (TNFAIP6) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNFAIP6. No significant cross-reactivity or interference between Human TNFAIP6 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TSG-6 is a 30 kDa secreted protein that contains a hyaluronan-binding LINK domain a and thus is a member of the hyaluronan-binding protein family, also called hyaladherins. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable, covalent complex with inter-alpha-inhibitor (IalphaI), and thus enhance the serine protease inhibitory activity of IalphaI, which is important in the protease network associated with inflammation. The expression of this gene can be induced by a number of signalling molecules, principally tumor necrosis factor alpha (TNF-alpha) and interleukin-1 (IL-1). The expression can also be induced by mechanical stimuli in vascular smooth muscle cells, and is found to be correlated with proteoglycan synthesis and aggregation.
Notes	This Human Tumor necrosis factor-inducible gene 6 protein (TNFAIP6) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFAIP6 in samples. An antibody specific for TNFAIP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFAIP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFAIP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFAIP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	7130

Applications	ELISA
Applications Notes	This Human Tumor necrosis factor-inducible gene 6 protein (TNFAIP6) ELISA Kit employs a two-site sandwich ELISA to quantitate TNFAIP6 in samples. An antibody specific for TNFAIP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNFAIP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNFAIP6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNFAIP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702451	KTE60233	48 T	$339		Add to Cart
BHE13702451	KTE60233	96 T	$589		Add to Cart
BHE13702451	KTE60233	96 Tx5	$2629		Add to Cart
BHE13702451	KTE60233	96 Tx50	$24569		Add to Cart

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