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Home / ELISA Kit

Human Tubulin polymerization-promoting protein (TPPP) ELISA Kit

SKU : BHE13702363

Seki et al. (1999) cloned p25 from a neuroblastoma cDNA library. The deduced 219-amino acid protein has a calculated molecular mass of about 24 kD. Human and bovine p25 share 90% amino acid identity. In contrast to the brain-specific expression of bovine p25, RT-PCR detected human p25 in all tissues examined.Martin et al. (2002) found that p24 associated with Gsk3 in microtubules from adult rat brain. p24 bound Gsk3 and inhibited its kinase activity at low magnesium concentrations. p24 was a poor substrate for Gsk3, but it could be phosphorylated by other protein kinases. Bovine Tppp inhibited mitotic spindle assembly and nuclear envelope breakdown following injection into Drosophila embryos. It did not affect other cellular events.

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Mfr.No.	KTE60144
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Tubulin polymerization-promoting protein (TPPP) ELISA Kit employs a two-site sandwich ELISA to quantitate TPPP.
Alternative Names	TPPP; TPPP/p25; TPPP1; p24; p25; p25alpha; brain specific protein p25 alpha; glycogen synthase kinase 3 (GSK3) inhibitor p24; tubulin polymerization-promoting protein
Detection Method	Colorimetric
Kit Components	Human Tubulin polymerization-promoting protein microplate Human Tubulin polymerization-promoting protein standard Human Tubulin polymerization-promoting protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Tubulin polymerization-promoting protein (TPPP) ELISA Kit has high sensitivity and excellent specificity for detection of Human TPPP. No significant cross-reactivity or interference between Human TPPP and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Seki et al. (1999) cloned p25 from a neuroblastoma cDNA library. The deduced 219-amino acid protein has a calculated molecular mass of about 24 kD. Human and bovine p25 share 90% amino acid identity. In contrast to the brain-specific expression of bovine p25, RT-PCR detected human p25 in all tissues examined.Martin et al. (2002) found that p24 associated with Gsk3 in microtubules from adult rat brain. p24 bound Gsk3 and inhibited its kinase activity at low magnesium concentrations. p24 was a poor substrate for Gsk3, but it could be phosphorylated by other protein kinases. Bovine Tppp inhibited mitotic spindle assembly and nuclear envelope breakdown following injection into Drosophila embryos. It did not affect other cellular events.
Notes	This Human Tubulin polymerization-promoting protein (TPPP) ELISA Kit employs a two-site sandwich ELISA to quantitate TPPP in samples. An antibody specific for TPPP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPPP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPPP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPPP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	11076

Applications	ELISA
Applications Notes	This Human Tubulin polymerization-promoting protein (TPPP) ELISA Kit employs a two-site sandwich ELISA to quantitate TPPP in samples. An antibody specific for TPPP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPPP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPPP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPPP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702363	KTE60144	48 T	$339		Add to Cart
BHE13702363	KTE60144	96 T	$589		Add to Cart
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BHE13702363	KTE60144	96 Tx50	$24569		Add to Cart

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