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Home / ELISA Kit

Human Tubulin beta-4 chain (TUBB4) ELISA Kit

SKU : BHE13702335

TUBB3 expression in several human cell lines, but not in a paclitaxel-resistant cell line. RNA interference revealed that HIF1A mediated hypoxia-induced TUBB3 expression. Chromatin immunoprecipitation analysis showed that HIF1A bound an HIF1A-binding site in the 5-prime flanking region of the TUBB3 gene. Methylation of an enhancer in the 3-prime flanking region abolished hypoxia-induced TUBB3 expression. Much lower Tubb3 levels were detected in all other adult mouse tissues examined except brain, where expression was even lower. Chromatin immunoprecipitation analysis showed that HIF1A bound an HIF1A-binding site in the 5-prime flanking region of the TUBB3 gene. Methylation of an enhancer in the 3-prime flanking region abolished hypoxia-induced TUBB3 expression.

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Mfr.No.	KTE60116
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Tubulin beta-4 chain (TUBB4) ELISA Kit employs a two-site sandwich ELISA to quantitate TUBB4.
Alternative Names	TUBB4; TUBB5; beta-5; tubulin; beta; 5
Detection Method	Colorimetric
Kit Components	Human Tubulin beta-4 chain microplate Human Tubulin beta-4 chain standard Human Tubulin beta-4 chain detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Tubulin beta-4 chain (TUBB4) ELISA Kit has high sensitivity and excellent specificity for detection of Human TUBB4. No significant cross-reactivity or interference between Human TUBB4 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TUBB3 expression in several human cell lines, but not in a paclitaxel-resistant cell line. RNA interference revealed that HIF1A mediated hypoxia-induced TUBB3 expression. Chromatin immunoprecipitation analysis showed that HIF1A bound an HIF1A-binding site in the 5-prime flanking region of the TUBB3 gene. Methylation of an enhancer in the 3-prime flanking region abolished hypoxia-induced TUBB3 expression. Much lower Tubb3 levels were detected in all other adult mouse tissues examined except brain, where expression was even lower. Chromatin immunoprecipitation analysis showed that HIF1A bound an HIF1A-binding site in the 5-prime flanking region of the TUBB3 gene. Methylation of an enhancer in the 3-prime flanking region abolished hypoxia-induced TUBB3 expression.
Notes	This Human Tubulin beta-4 chain (TUBB4) ELISA Kit employs a two-site sandwich ELISA to quantitate TUBB4 in samples. An antibody specific for TUBB4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTUBB4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TUBB4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TUBB4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	10382

Applications	ELISA
Applications Notes	This Human Tubulin beta-4 chain (TUBB4) ELISA Kit employs a two-site sandwich ELISA to quantitate TUBB4 in samples. An antibody specific for TUBB4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTUBB4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TUBB4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TUBB4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702335	KTE60116	48 T	$339		Add to Cart
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BHE13702335	KTE60116	96 Tx50	$24569		Add to Cart

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