Human Tubulin Beta-3 Chain (TUBB3)ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14121h

Alternative Names

tubulin, beta 3, MC1R, TUBB4, beta-4, tubulin, beta, 4

Reactivity

Human

Detection Range

0.625 ng/mL-40 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human HSP-72 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	99	92-107

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human HSP-72 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-90
1:2	Average %	98
	Range %	91-103
1:4	Average %	101
	Range %	92-108
1:8	Average %	93
	Range %	86-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sensitivity

0.156 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q13509
Antigen	tubulin, beta 3

Applications

ELISA

Research Area

TUBB3

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10504711	CSB-E14121h	96T	$695	Add to Cart
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