Human Tubulin Beta-1 Chain - TUBB1 ELISA Kit

Mfr.No.

E1608Hu

Sensitivity

2.74ng/L

Detection Range

5-1000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Tubulin Beta-1 Chain (also known as TUBB1) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

TUBB 1;TUBB1;Tubulin beta-1 chain

Molecular Weight

50327

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E1608Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	336.8	17.7	5.3
Intra-Assay	2	18	489.1	16.52	3.4
Intra-Assay	3	18	195.9	9.13	4.7

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).

Protocol

These standard curves of E1608Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	1.75	1.772	1.715
	1.794
320ng/L	0.906	1.009	0.951
	1.112
160ng/L	0.553	0.574	0.517
	0.595
80ng/L	0.251	0.249	0.191
	0.247
40ng/L	0.106	0.092	0.034
	0.078
0ng/L	0.053	0.058	0
	0.062

Gene ID	81027
UniProt ID	Q9H4B7

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103195	E1608Hu	48T	$320		Add to Cart
BHE12103195	E1608Hu	96T	$458		Add to Cart

Human Tubulin Beta-1 Chain - TUBB1 ELISA Kit

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