Matrices listed below were spiked with certain level of TUBG1 and the recovery rates were calculated by comparing the measured value to the expected amount of TUBG1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-103
92
EDTA plasma(n=5)
87-105
95
heparin plasma(n=5)
86-105
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TUBG1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-105%
93-103%
95-105%
EDTA plasma(n=5)
83-98%
89-101%
93-98%
heparin plasma(n=5)
88-100%
83-98%
80-96%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
P23258
Applications
ELISA
Applications Notes
TUBG1 ELISA Kit allows for the in vitro quantitative determination of TUBG1 concentrations in serum, plasma, tissue homogenates and other biological fluids.