Matrices listed below were spiked with certain level of TSPAN4 and the recovery rates were calculated by comparing the measured value to the expected amount of TSPAN4 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
89-105
98
EDTA plasma(n=5)
88-104
94
heparin plasma(n=5)
87-100
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TSPAN4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-105%
87-101%
94-102%
EDTA plasma(n=5)
87-96%
89-100%
86-101%
heparin plasma(n=5)
88-98%
86-99%
90-100%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
O14817
Applications
ELISA
Applications Notes
TSPAN4 ELISA Kit allows for the in vitro quantitative determination of TSPAN4 concentrations in serum, plasma, tissue homogenates and other biological fluids.