Matrices listed below were spiked with certain level of TSPAN12 and the recovery rates were calculated by comparing the measured value to the expected amount of TSPAN12 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-102
96
EDTA plasma(n=5)
90-104
96
heparin plasma(n=5)
85-105
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TSPAN12 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-87%
87-103%
91-105%
EDTA plasma(n=5)
83-99%
84-97%
83-99%
heparin plasma(n=5)
80-94%
81-100%
81-100%
Sensitivity
< 46.875pg/ml
Notes
For Research Use Only
UniProt ID
O95859
Applications
ELISA
Applications Notes
TSPAN12 ELISA Kit allows for the in vitro quantitative determination of TSPAN12 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human TSPAN12(Tetraspanin-12) ELISA Kit