Human TSP2/THBS2/Thrombospondin-2 ELISA Kit PicoKine®

Mfr.No.

EK0642

Sensitivity

<10 pg/ml

Detection Range

625 pg/ml - 40,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	625	1250	2500	5000	10000	20000	40000
O.D.	0.025	0.110	0.191	0.343	0.605	0.977	1.630	2.067

Description

Human TSP2 ELISA Kit PicoKine® (96 Tests). Quantitate Human THBS2 in cell culture supeatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Thrombospondin-2;THBS2;TSP2;

Molecular Weight

129991 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: G19-I1172

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TSP2

Sample Type

Cell culture supeatants, Serum, Plasma, Human milk

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	234	1702	6045	222	1773	6515
Standard deviation	17.55	789	411.06	17.76	117.54	501.65
CV (%)	7.5%	4.6%	6.8%	8%	6.5%	7.7%

Protein Function

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Ligand for CD36 mediating antiangiogenic properties. .

Background

Thrombospondin-2 (TSP-2) is a protein that in humans is encoded by the THBS2 gene.And this gene is mapped to 6q27. The protein encoded by this gene belongs to the thrombospondin family. It is a disulfide-linked homotrimeric glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. This protein has been shown to function as a potent inhibitor of tumor growth and angiogenesis. Studies of the mouse counterpart suggest that this protein may modulate the cell surface properties of mesenchymal cells and be involved in cell adhesion and migration. TSP-2 has been shown to interact with MMP2.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	234	245	217	210	226	13.79	6.1%
Sample 2	1702	1693	1719	1783	1724	35.18	2%
Sample 3	6045	5875	6096	5562	5894	208.67	3.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	7058
Gene Name	THBS2
UniProt ID	P35442
Protein Name	Thrombospondin-2
Tissue Specificity	High expression in invertebral disk tissue. .
Sequence	Belongs to the thrombospondin family.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000545	EK0642	96 wells/kit	$499		Add to Cart

Human TSP2/THBS2/Thrombospondin-2 ELISA Kit PicoKine®

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