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Home / ELISA Kit

Human TSC22 domain family protein 3 (TSC22D3) ELISA Kit

SKU : BHE13702343

Delta sleep-inducing peptide, abbreviated DSIP, is a neuropeptide that when infused into the mesodiencephalic ventricle of recipient rabbits induces spindle and delta EEG activity and reduced motor activities. DSIP was discovered by G.Schoeneberger and M.Monnier in 1974; first research aimed at it's somnogenous properties lead to the dubious results. Instead it was demonstrated by the works of different teams that DSIP actually plays a important role in the stress-resistance. DSIP has stress-protective, antiseizure, and immunomodulating effects. It has been demonstrated that DSIP have significant geroprotective effect. K.V. Sudakov views DSIP as one of the 4 main substances, responsible for the stress-resistance of the organism, another 3 being substance P, prolactin and beta-endorphin.

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Mfr.No.	KTE60124
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human TSC22 domain family protein 3 (TSC22D3) ELISA Kit employs a two-site sandwich ELISA to quantitate TSC22D3.
Alternative Names	TSC22D3; RP13-364K23.1; DIP; DKFZp313A1123; DSIPI; GILZ; TSC-22R; hDIP; DSIP-immunoreactive leucine zipper protein; OTTHUMP00000023814; TSC-22 related protein; delta sleep inducing peptide; immunoreactor; glucoc
Detection Method	Colorimetric
Kit Components	Human TSC22 domain family protein 3 microplate Human TSC22 domain family protein 3 standard Human TSC22 domain family protein 3 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human TSC22 domain family protein 3 (TSC22D3) ELISA Kit has high sensitivity and excellent specificity for detection of Human TSC22D3. No significant cross-reactivity or interference between Human TSC22D3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Delta sleep-inducing peptide, abbreviated DSIP, is a neuropeptide that when infused into the mesodiencephalic ventricle of recipient rabbits induces spindle and delta EEG activity and reduced motor activities. DSIP was discovered by G.Schoeneberger and M.Monnier in 1974; first research aimed at it's somnogenous properties lead to the dubious results. Instead it was demonstrated by the works of different teams that DSIP actually plays a important role in the stress-resistance. DSIP has stress-protective, antiseizure, and immunomodulating effects. It has been demonstrated that DSIP have significant geroprotective effect. K.V. Sudakov views DSIP as one of the 4 main substances, responsible for the stress-resistance of the organism, another 3 being substance P, prolactin and beta-endorphin.
Notes	This Human TSC22 domain family protein 3 (TSC22D3) ELISA Kit employs a two-site sandwich ELISA to quantitate TSC22D3 in samples. An antibody specific for TSC22D3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTSC22D3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSC22D3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSC22D3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	1831

Applications	ELISA
Applications Notes	This Human TSC22 domain family protein 3 (TSC22D3) ELISA Kit employs a two-site sandwich ELISA to quantitate TSC22D3 in samples. An antibody specific for TSC22D3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTSC22D3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TSC22D3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TSC22D3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702343	KTE60124	48 T	$339		Add to Cart
BHE13702343	KTE60124	96 T	$589		Add to Cart
BHE13702343	KTE60124	96 Tx5	$2629		Add to Cart
BHE13702343	KTE60124	96 Tx50	$24569		Add to Cart

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