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Home / ELISA Kit

Human Tryptophan Hydroxylase 1 - TPH1 ELISA Kit

SKU : BHE12104628

Oxidizes L-tryptophan to 5-hydroxy-l-tryptophan in the rate-determining step of serotonin biosynthesis.

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Bioassay Technology Laboratory

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+1 866.986.9598

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Mfr.No.

E3095Hu

Sensitivity

4.04ng/L

Detection Range

10-2000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

TPH1;TPRH;TRPH;Tryptophan 5-hydroxylase 1;Tryptophan 5-monooxygenase 1

Molecular Weight

50985

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E3095Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	266.44	11.68	4.38
Intra-Assay	2	18	792.21	42.66	5.38
Intra-Assay	3	18	1226.72	44.69	3.64

Background

Oxidizes L-tryptophan to 5-hydroxy-l-tryptophan in the rate-determining step of serotonin biosynthesis.

Protocol

These standard curves of E3095Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200ng/L	1.758	1.793	1.739
	1.828
600ng/L	0.964	0.957	0.903
	0.951
300ng/L	0.547	0.548	0.494
	0.549
150ng/L	0.261	0.229	0.175
	0.198
75ng/L	0.107	0.115	0.061
	0.124
0ng/L	0.049	0.054	0
	0.06

Gene ID	7166
UniProt ID	P17752

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104628	E3095Hu	48T	$320		Add to Cart
BHE12104628	E3095Hu	96T	$458		Add to Cart

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