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Home / ELISA Kit

Human Tryptase Delta - TPSD1 ELISA Kit

SKU : BHE12107691

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.

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Bioassay Technology Laboratory

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Mfr.No.

E6329Hu

Sensitivity

0.022ng/mL

Detection Range

0.05-20ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Delta-tryptase;HmMCP-3-like tryptase III;Mast cell mMCP-7-like;MCP7L1;MCP7-LIKE;MMCP-7L;TPSD1;Tryptase delta;Tryptase-3;

Molecular Weight

26584

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E6329Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.83	0.25	5.19
Intra-Assay	2	18	5.96	0.18	3.07
Intra-Assay	3	18	1.83	0.09	4.76

Background

Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type.

Protocol

These standard curves of E6329Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	2.292	2.176	2.14
	2.061
6ng/mL	1.407	1.447	1.411
	1.488
3ng/mL	0.595	0.687	0.651
	0.779
1.5ng/mL	0.349	0.378	0.342
	0.408
0.75ng/mL	0.175	0.169	0.133
	0.163
0ng/mL	0.043	0.036	0
	0.029

Gene ID	23430
UniProt ID	Q9BZJ3

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107691	E6329Hu	48T	$320		Add to Cart
BHE12107691	E6329Hu	96T	$458		Add to Cart

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