Human Troponin I, slow skeletal muscle(TNNI1) ELISA kit

Human Troponin I, slow skeletal muscle(TNNI1) ELISA kit

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Mfr.No.

CSB-EL024011HU

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

troponin I type 1 (skeletal, slow), DKFZp451O223, SSTNI, TNN1, OTTHUMP00000033877, OTTHUMP00000033910, slow-twitch skeletal troponin I, troponin I, skeletal, slow

Recovery

The recovery of mouse SORT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	96	92-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse SORT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-102
1:2	Average %	94
	Range %	88-98
1:4	Average %	100
	Range %	96-105
1:8	Average %	92
	Range %	86-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
400	2.576	2.502	2.539	2.376
200	2.107	2.006	2.057	1.894
100	1.423	1.364	1.394	1.231
50	0.729	0.730	0.730	0.567
25	0.470	0.434	0.452	0.289
12.5	0.326	0.306	0.316	0.153
6.25	0.262	0.247	0.255	0.092
0	0.157	0.168	0.163	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P19237

Applications	ELISA
Research Area	TNNI1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504695	CSB-EL024011HU	96T	$595	Add to Cart
BHE10504695	CSB-EL024011HU	5x96T	$2856	Add to Cart
BHE10504695	CSB-EL024011HU	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.