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Home / ELISA Kit

Human Tropomyosin alpha-3 chain (TPM3) ELISA Kit

SKU : BHE13702368

Tropomyosin alpha-3 chain is a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

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Mfr.No.	KTE60149
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Tropomyosin alpha-3 chain (TPM3) ELISA Kit employs a two-site sandwich ELISA to quantitate TPM3.
Alternative Names	TPM3; RP11-205M9.1; FLJ41118; MGC14582; MGC3261; MGC72094; NEM1; OK/SW-cl.5; TM-5; TM3; TM30; TM30nm; TPMsk3; TRK; hscp30; cytoskeletal tropomyosin TM30; heat-stable cytoskeletal protein 30 kDa; tropomyosin
Detection Method	Colorimetric
Kit Components	Human Tropomyosin alpha-3 chain microplate Human Tropomyosin alpha-3 chain standard Human Tropomyosin alpha-3 chain detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Tropomyosin alpha-3 chain (TPM3) ELISA Kit has high sensitivity and excellent specificity for detection of Human TPM3. No significant cross-reactivity or interference between Human TPM3 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Tropomyosin alpha-3 chain is a member of the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins that polymerize end-to-end along the major groove in most actin filaments. They provide stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, they regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Mutations in this gene result in autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are associated with cancer. Multiple transcript variants encoding different isoforms have been found for this gene.
Notes	This Human Tropomyosin alpha-3 chain (TPM3) ELISA Kit employs a two-site sandwich ELISA to quantitate TPM3 in samples. An antibody specific for TPM3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPM3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPM3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPM3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	7170

Applications	ELISA
Applications Notes	This Human Tropomyosin alpha-3 chain (TPM3) ELISA Kit employs a two-site sandwich ELISA to quantitate TPM3 in samples. An antibody specific for TPM3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTPM3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TPM3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TPM3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702368	KTE60149	48 T	$339		Add to Cart
BHE13702368	KTE60149	96 T	$589		Add to Cart
BHE13702368	KTE60149	96 Tx5	$2629		Add to Cart
BHE13702368	KTE60149	96 Tx50	$24569		Add to Cart

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