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Home / ELISA Kit

Human Trinucleotide repeat-containing gene 6A protein (TNRC6A) ELISA Kit

SKU : BHE13702396

Trinucleotide repeat-containing gene 6A protein is a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing. Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins.

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Mfr.No.	KTE60177
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Trinucleotide repeat-containing gene 6A protein (TNRC6A) ELISA Kit employs a two-site sandwich ELISA to quantitate TNRC6A.
Alternative Names	TNRC6A; CAGH26; DKFZp666E117; FLJ22043; GW1; GW182; KIAA1460; MGC75384; TNRC6; CAG repeat protein 26; EDIE; GW182 autoantigen; glycine-tryptophan protein of 182 kDa; trinucleotide repeat-containing gene 6A
Detection Method	Colorimetric
Kit Components	Human Trinucleotide repeat-containing gene 6A protein microplate Human Trinucleotide repeat-containing gene 6A protein standard Human Trinucleotide repeat-containing gene 6A protein detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Trinucleotide repeat-containing gene 6A protein (TNRC6A) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNRC6A. No significant cross-reactivity or interference between Human TNRC6A and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Trinucleotide repeat-containing gene 6A protein is a member of the trinucleotide repeat containing 6 protein family. The protein functions in post-transcriptional gene silencing through the RNA interference (RNAi) and microRNA pathways. The protein associates with messenger RNAs and Argonaute proteins in cytoplasmic bodies known as GW-bodies or P-bodies. Inhibiting expression of this gene delocalizes other GW-body proteins and impairs RNAi and microRNA-induced gene silencing. Plays a role in RNA-mediated gene silencing by both micro-RNAs (miRNAs) and short interfering RNAs (siRNAs). Required for miRNA-dependent repression of translation and for siRNA-dependent endonucleolytic cleavage of complementary mRNAs by argonaute family proteins.
Notes	This Human Trinucleotide repeat-containing gene 6A protein (TNRC6A) ELISA Kit employs a two-site sandwich ELISA to quantitate TNRC6A in samples. An antibody specific for TNRC6A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNRC6A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNRC6A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNRC6A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	27327

Applications	ELISA
Applications Notes	This Human Trinucleotide repeat-containing gene 6A protein (TNRC6A) ELISA Kit employs a two-site sandwich ELISA to quantitate TNRC6A in samples. An antibody specific for TNRC6A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNRC6A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNRC6A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNRC6A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702396	KTE60177	48 T	$339		Add to Cart
BHE13702396	KTE60177	96 T	$589		Add to Cart
BHE13702396	KTE60177	96 Tx5	$2629		Add to Cart
BHE13702396	KTE60177	96 Tx50	$24569		Add to Cart

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