Matrices listed below were spiked with certain level of TRIM31 and the recovery rates were calculated by comparing the measured value to the expected amount of TRIM31 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-100
94
EDTA plasma(n=5)
90-102
97
heparin plasma(n=5)
91-102
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRIM31 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
91-103%
85-102%
91-104%
EDTA plasma(n=5)
84-97%
85-99%
83-99%
heparin plasma(n=5)
80-99%
86-100%
84-99%
Sensitivity
< 18.75pg/ml
Notes
For Research Use Only
UniProt ID
Q9BZY9
Applications
ELISA
Applications Notes
TRIM31 ELISA Kit allows for the in vitro quantitative determination of TRIM31 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human TRIM31(E3 ubiquitin-protein ligase TRIM31) ELISA Kit