Matrices listed below were spiked with certain level of TRIM29 and the recovery rates were calculated by comparing the measured value to the expected amount of TRIM29 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-104
94
EDTA plasma(n=5)
85-102
93
heparin plasma(n=5)
85-101
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of TRIM29 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
85-102%
80-104%
88-100%
EDTA plasma(n=5)
91-99%
91-100%
91-98%
heparin plasma(n=5)
93-96%
90-99%
91-98%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
Q14134
Applications
ELISA
Applications Notes
TRIM29 ELISA Kit allows for the in vitro quantitative determination of TRIM29 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Human TRIM29(Tripartite motif-containing protein 29) ELISA Kit