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Home / ELISA Kit

Human Tri-iodothyronine (T3) ELISA Kit

SKU : BHE13702667

Triiodothyronine, also known as T?, is a thyroid hormone. It affects almost every physiological process in the body, including growth and development, metabolism, body temperature, and heart rate.

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTE60450
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Tri-iodothyronine (T3) ELISA Kit employs a two-site sandwich ELISA to quantitate T3.
Alternative Names	T3
Detection Method	Colorimetric
Kit Components	Human Tri-iodothyronine microplate Human Tri-iodothyronine standard Human Tri-iodothyronine detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Tri-iodothyronine (T3) ELISA Kit has high sensitivity and excellent specificity for detection of Human T3. No significant cross-reactivity or interference between Human T3 and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supeatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Triiodothyronine, also known as T?, is a thyroid hormone. It affects almost every physiological process in the body, including growth and development, metabolism, body temperature, and heart rate.
Notes	This Human Tri-iodothyronine (T3) ELISA Kit employs a two-site sandwich ELISA to quantitate T3 in samples. An antibody specific for T3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for T3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of T3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Tri-iodothyronine (T3) ELISA Kit employs a two-site sandwich ELISA to quantitate T3 in samples. An antibody specific for T3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for T3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of T3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702667	KTE60450	48 T	$259		Add to Cart
BHE13702667	KTE60450	96 T	$469		Add to Cart
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BHE13702667	KTE60450	96 Tx50	$18719		Add to Cart

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