Human Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine®

Mfr.No.

EK1684

Sensitivity

<10 pg/ml

Detection Range

312 pg/ml - 20,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	312	624	1250	2500	5000	10000	20000
O.D.	0.009	0.059	0.168	0.352	0.560	1.104	1.664	1.897

Description

Human Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine® (96 Tests). Quantitate Human TTR in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml . The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Transthyretin; ATTR; Prealbumin; TBPA; TTR; PALB

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G21-E147

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human TTR

Subcellular Localization

Secreted. Cytoplasm.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	456	1624	9289	529	1947	9298
Standard deviation	34.34	55.87	599.02	33.49	38.94	304.04
CV (%)	7.5%	3.4%	6.4%	6.3%	2%	3.3%

Protein Function

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.

Background

Transthyretin (TTR) is also known as prealbumin. It is mapped to chromosome region 18q11.2-q12.1. This gene encodes a carrier protein responsible for the transport of thyroid hormones and retinol. The protein consists of a tetramer of identical subunits. Due to increased stability of the tetramer form of this encoded protein in mouse, compared to the human protein, this gene product has a reduced tendency to form amyloid fibrils. In humans, this protein binds beta-amyloid preventing its aggregation and providing a neuroprotective role in Alzheimer's disease.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	456	596	343	482	469	35.71	7.6%
Sample 2	1624	977	1230	767	1150	82.99	7.2%
Sample 3	9389	6426	8298	6358	7618	509.63	6.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	7276
Gene Name	TTR
UniProt ID	P02766
Tissue Specificity	Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.

Applications	ELISA
Research Area	Alzheimer's Disease,Cardiovascular,Neurodegenerative Disease,Neurology Process,Neuroscience,Serum Proteins

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001346	EK1684	96 wells/kit	$750		Add to Cart

Human Transthyretin / TTR / PreAlbumin ELISA Kit PicoKine®

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