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Home / ELISA Kit

Human Transportin-2 (TNPO2) ELISA Kit

SKU : BHE13702401

Transportin-2 (KPNB2) interacts directly and specifically with M9, the bidirectional transport signal of the nuclear shuttling protein hnRNPA1 and mediates hnRNPA1 nuclear import. In the course of isolating additional transportin-1 cDNAs, Siomi et al. (1997) isolated cDNAs encoding a related protein that they designated 'transportin-2.' The sequence of the predicted 894-amino acid protein shares 84% identity with that of transportin-1. One notable difference is that transportin-2 contains a short extra sequence within the region corresponding to the M9-interacting domain of transportin-1. Far Western blotting showed that transportin-2 and transportin-1 have different substrate specificities. Siomi et al. (1997) suggested that the insert in transportin-2 modifies its interaction with import substrates.

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Mfr.No.	KTE60182
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Transportin-2 (TNPO2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNPO2.
Alternative Names	TNPO2; FLJ12155; IPO3; KPNB2B; TRN2; importin 3; karyopherin beta 2b; transportin; transportin 2 (importin 3; karyopherin beta 2b)
Detection Method	Colorimetric
Kit Components	Human Transportin-2 microplate Human Transportin-2 standard Human Transportin-2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Transportin-2 (TNPO2) ELISA Kit has high sensitivity and excellent specificity for detection of Human TNPO2. No significant cross-reactivity or interference between Human TNPO2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Transportin-2 (KPNB2) interacts directly and specifically with M9, the bidirectional transport signal of the nuclear shuttling protein hnRNPA1 and mediates hnRNPA1 nuclear import. In the course of isolating additional transportin-1 cDNAs, Siomi et al. (1997) isolated cDNAs encoding a related protein that they designated 'transportin-2.' The sequence of the predicted 894-amino acid protein shares 84% identity with that of transportin-1. One notable difference is that transportin-2 contains a short extra sequence within the region corresponding to the M9-interacting domain of transportin-1. Far Western blotting showed that transportin-2 and transportin-1 have different substrate specificities. Siomi et al. (1997) suggested that the insert in transportin-2 modifies its interaction with import substrates.
Notes	This Human Transportin-2 (TNPO2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNPO2 in samples. An antibody specific for TNPO2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNPO2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNPO2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNPO2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	30000

Applications	ELISA
Applications Notes	This Human Transportin-2 (TNPO2) ELISA Kit employs a two-site sandwich ELISA to quantitate TNPO2 in samples. An antibody specific for TNPO2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTNPO2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNPO2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNPO2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702401	KTE60182	48 T	$339		Add to Cart
BHE13702401	KTE60182	96 T	$589		Add to Cart
BHE13702401	KTE60182	96 Tx5	$2629		Add to Cart
BHE13702401	KTE60182	96 Tx50	$24569		Add to Cart

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