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Home / ELISA Kit

Human Transmembrane protein 63B (TMEM63B) ELISA Kit

SKU : BHE13702487

TMEM63B belongs to the SPO75/TMEM63 family. 3 isoforms of the human protein are produced by alternative splicing. Membrane; Multi-pass membrane protein, The exact function of TMEM63A remains unknown. spo75 mutants displayed a very heterogeneous phenotype. The phenotypes of spores in spo75 cells ranged from those that had little or no spore wall material to ones that had a wild-type appearance.
A transmembrane protein is a polytopic protein that spans the entire biological membrane. Transmembrane proteins aggregate and precipitate in water. They require detergents or nonpolar solvents for extraction, although some of them (beta-barrels) can be also extracted using denaturing agents.

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Mfr.No.	KTE60269
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Transmembrane protein 63B (TMEM63B) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM63B.
Alternative Names	TMEM63B; C6orf110; DKFZp686N2275; RP3-421H19.2; OTTHUMP00000016502
Detection Method	Colorimetric
Kit Components	Human Transmembrane protein 63B microplate Human Transmembrane protein 63B standard Human Transmembrane protein 63B detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Transmembrane protein 63B (TMEM63B) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM63B. No significant cross-reactivity or interference between Human TMEM63B and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TMEM63B belongs to the SPO75/TMEM63 family. 3 isoforms of the human protein are produced by alternative splicing. Membrane; Multi-pass membrane protein, The exact function of TMEM63A remains unknown. spo75 mutants displayed a very heterogeneous phenotype. The phenotypes of spores in spo75 cells ranged from those that had little or no spore wall material to ones that had a wild-type appearance. A transmembrane protein is a polytopic protein that spans the entire biological membrane. Transmembrane proteins aggregate and precipitate in water. They require detergents or nonpolar solvents for extraction, although some of them (beta-barrels) can be also extracted using denaturing agents.
Notes	This Human Transmembrane protein 63B (TMEM63B) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM63B in samples. An antibody specific for TMEM63B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM63B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM63B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM63B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	55362

Applications	ELISA
Applications Notes	This Human Transmembrane protein 63B (TMEM63B) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM63B in samples. An antibody specific for TMEM63B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM63B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM63B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM63B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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