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Home / ELISA Kit

Human Transmembrane protein 54 (TMEM54) ELISA Kit

SKU : BHE13702497

Ubiquitously expressed in cancer cell lines.Cloning and characterization of a cDNA fragment coding beta-casein-like protein preferentially expressed in cervical adenocarcinoma cell line CAC-1.
One of eight new monoclonal antibodies generated, 12G2, specifically reacted not only with beta-casein, but also with human cervical adenocarcinoma cell line CAC-1. To identify the gene encoding the putative protein (BCLP) defined by monoclonal antibody (mAb) 12G2, a complementary DNA library was constructed. the inserted cDNA fragment that showed high homology to reported DNA sequences of unknown function in normal human placenta. BCLP was overexpressed or changed its structure by carcinogenesis.

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Mfr.No.	KTE60279
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Transmembrane protein 54 (TMEM54) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM54.
Alternative Names	TMEM54; BCLP; CAC-1; CAC1; MGC10137; beta-casein-like protein
Detection Method	Colorimetric
Kit Components	Human Transmembrane protein 54 microplate Human Transmembrane protein 54 standard Human Transmembrane protein 54 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Transmembrane protein 54 (TMEM54) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM54. No significant cross-reactivity or interference between Human TMEM54 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Ubiquitously expressed in cancer cell lines.Cloning and characterization of a cDNA fragment coding beta-casein-like protein preferentially expressed in cervical adenocarcinoma cell line CAC-1. One of eight new monoclonal antibodies generated, 12G2, specifically reacted not only with beta-casein, but also with human cervical adenocarcinoma cell line CAC-1. To identify the gene encoding the putative protein (BCLP) defined by monoclonal antibody (mAb) 12G2, a complementary DNA library was constructed. the inserted cDNA fragment that showed high homology to reported DNA sequences of unknown function in normal human placenta. BCLP was overexpressed or changed its structure by carcinogenesis.
Notes	This Human Transmembrane protein 54 (TMEM54) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM54 in samples. An antibody specific for TMEM54 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM54 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM54 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM54 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	113452

Applications	ELISA
Applications Notes	This Human Transmembrane protein 54 (TMEM54) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM54 in samples. An antibody specific for TMEM54 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM54 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM54 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM54 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702497	KTE60279	48 T	$339		Add to Cart
BHE13702497	KTE60279	96 T	$589		Add to Cart
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BHE13702497	KTE60279	96 Tx50	$24569		Add to Cart

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