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Home / ELISA Kit

Human Transmembrane emp24 domain-containing protein 9 (TMED9) ELISA Kit

SKU : BHE13702518

TMED9 Belongs to the EMP24/GP25L family.Contains 1 GOLD domain. Members of the p24 (p24/gp25L/emp24/Erp) family of proteins have been shown to be critical components of the coated vesicles that are involved in the transportation of cargo molecules from the endoplasmic reticulum to the Golgi complex. The p24 proteins form hetero-oligomeric complexes and are believed to function as receptors for specific secretory cargo. The identification of the GOLD domain could aid in directed investigation of the role of the p24 proteins in the secretion process. The newly detected group of GOLD-domain proteins, which might simultaneously bind membranes and other proteins, point to the existence of a novel class of adaptors that could have a role in the assembly of membrane-associated complexes or in regulating assembly of cargo into membranous vesicles.

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Mfr.No.	KTE60300
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Transmembrane emp24 domain-containing protein 9 (TMED9) ELISA Kit employs a two-site sandwich ELISA to quantitate TMED9.
Alternative Names	TMED9; HSGP25L2G; gp25L2 protein
Detection Method	Colorimetric
Kit Components	Human Transmembrane emp24 domain-containing protein 9 microplate Human Transmembrane emp24 domain-containing protein 9 standard Human Transmembrane emp24 domain-containing protein 9 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Transmembrane emp24 domain-containing protein 9 (TMED9) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMED9. No significant cross-reactivity or interference between Human TMED9 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TMED9 Belongs to the EMP24/GP25L family.Contains 1 GOLD domain. Members of the p24 (p24/gp25L/emp24/Erp) family of proteins have been shown to be critical components of the coated vesicles that are involved in the transportation of cargo molecules from the endoplasmic reticulum to the Golgi complex. The p24 proteins form hetero-oligomeric complexes and are believed to function as receptors for specific secretory cargo. The identification of the GOLD domain could aid in directed investigation of the role of the p24 proteins in the secretion process. The newly detected group of GOLD-domain proteins, which might simultaneously bind membranes and other proteins, point to the existence of a novel class of adaptors that could have a role in the assembly of membrane-associated complexes or in regulating assembly of cargo into membranous vesicles.
Notes	This Human Transmembrane emp24 domain-containing protein 9 (TMED9) ELISA Kit employs a two-site sandwich ELISA to quantitate TMED9 in samples. An antibody specific for TMED9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMED9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMED9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMED9 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	54732

Applications	ELISA
Applications Notes	This Human Transmembrane emp24 domain-containing protein 9 (TMED9) ELISA Kit employs a two-site sandwich ELISA to quantitate TMED9 in samples. An antibody specific for TMED9 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMED9 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMED9 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMED9 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702518	KTE60300	48 T	$339		Add to Cart
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BHE13702518	KTE60300	96 Tx50	$24569		Add to Cart

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