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Home / ELISA Kit

Human Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) ELISA Kit

SKU : BHE13702462

Tmub1 encodes for a protein, HOPS/DULP, containing transmembrane domains and a ubiquitin-like domain (UBL) Ubiquitin can be employed to modify proteins by either single or multiple ubiquitin conjugation to mark them for proteasomal destruction, endocytosis and binding to other proteins. Tmub1/HOPS was reported to facilitate the recycling of AMPA receptors into synaptic membrane in cultured primary neurons. Tmub1/HOPS was also found overexpressed during liver regeneration and is an essential constituent of centrosome assembly during cell cycles in culture. Like many UBL domain proteins, the physiological and molecular function of Tmub1 is far from being clear. The resulting mice Tmub1were put through an extensive battery of assays to discover physiological and behavioral phenotypes.

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Mfr.No.	KTE60244
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMUB1.
Alternative Names	TMUB1; C7orf21; MGC5442; SB144;
Detection Method	Colorimetric
Kit Components	Human Transmembrane and ubiquitin-like domain-containing protein 1 microplate Human Transmembrane and ubiquitin-like domain-containing protein 1 standard Human Transmembrane and ubiquitin-like domain-containing protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMUB1. No significant cross-reactivity or interference between Human TMUB1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Tmub1 encodes for a protein, HOPS/DULP, containing transmembrane domains and a ubiquitin-like domain (UBL) Ubiquitin can be employed to modify proteins by either single or multiple ubiquitin conjugation to mark them for proteasomal destruction, endocytosis and binding to other proteins. Tmub1/HOPS was reported to facilitate the recycling of AMPA receptors into synaptic membrane in cultured primary neurons. Tmub1/HOPS was also found overexpressed during liver regeneration and is an essential constituent of centrosome assembly during cell cycles in culture. Like many UBL domain proteins, the physiological and molecular function of Tmub1 is far from being clear. The resulting mice Tmub1were put through an extensive battery of assays to discover physiological and behavioral phenotypes.
Notes	This Human Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMUB1 in samples. An antibody specific for TMUB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMUB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMUB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMUB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	83590

Applications	ELISA
Applications Notes	This Human Transmembrane and ubiquitin-like domain-containing protein 1 (TMUB1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMUB1 in samples. An antibody specific for TMUB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMUB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMUB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMUB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702462	KTE60244	48 T	$339		Add to Cart
BHE13702462	KTE60244	96 T	$589		Add to Cart
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BHE13702462	KTE60244	96 Tx50	$24569		Add to Cart

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