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Home / ELISA Kit

Human Transmembrane and immunoglobulin domain-containing protein 1 (TMIGD1) ELISA Kit

SKU : BHE13702478

TMIGD2 Contains2 Ig-like (immunoglobulin-like) domain. Immunoglobulin-like domains that are related in both sequence and structure can be found in several diverse protein families. Ig-like domains are involved in a variety of functions, including cell-cell recognition, cell-surface receptors, muscle structure and the immune system. This entry is for immunoglobulin-like domains. Studies indicate that the interactions essential for defining the structure of these beta sandwich proteins are also important in nucleation of folding, and that proteins containing this fold may share similar folding pathways even though the proteins may have low sequence homology. The fold consists of a beta-sandwich formed of 7 strands in 2 sheets with a Greek-key topology. Some members of the fold have additional strands.

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Mfr.No.	KTE60260
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Transmembrane and immunoglobulin domain-containing protein 1 (TMIGD1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMIGD1.
Alternative Names	TMIGD1; TMIGD; UNQ9372; AWKS9372; transmembrane and immunoglobulin domain containing
Detection Method	Colorimetric
Kit Components	Human Transmembrane and immunoglobulin domain-containing protein 1 microplate Human Transmembrane and immunoglobulin domain-containing protein 1 standard Human Transmembrane and immunoglobulin domain-containing protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Transmembrane and immunoglobulin domain-containing protein 1 (TMIGD1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMIGD1. No significant cross-reactivity or interference between Human TMIGD1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TMIGD2 Contains2 Ig-like (immunoglobulin-like) domain. Immunoglobulin-like domains that are related in both sequence and structure can be found in several diverse protein families. Ig-like domains are involved in a variety of functions, including cell-cell recognition, cell-surface receptors, muscle structure and the immune system. This entry is for immunoglobulin-like domains. Studies indicate that the interactions essential for defining the structure of these beta sandwich proteins are also important in nucleation of folding, and that proteins containing this fold may share similar folding pathways even though the proteins may have low sequence homology. The fold consists of a beta-sandwich formed of 7 strands in 2 sheets with a Greek-key topology. Some members of the fold have additional strands.
Notes	This Human Transmembrane and immunoglobulin domain-containing protein 1 (TMIGD1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMIGD1 in samples. An antibody specific for TMIGD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMIGD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMIGD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMIGD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	388364

Applications	ELISA
Applications Notes	This Human Transmembrane and immunoglobulin domain-containing protein 1 (TMIGD1) ELISA Kit employs a two-site sandwich ELISA to quantitate TMIGD1 in samples. An antibody specific for TMIGD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMIGD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMIGD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMIGD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702478	KTE60260	48 T	$339		Add to Cart
BHE13702478	KTE60260	96 T	$589		Add to Cart
BHE13702478	KTE60260	96 Tx5	$2629		Add to Cart
BHE13702478	KTE60260	96 Tx50	$24569		Add to Cart

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