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Home / ELISA Kit

Human Translocating chain-associated membrane protein 1 (TRAM1) ELISA Kit

SKU : BHE13702356

TRAM1 is a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause. TRAM is a 374-amino acid, 8-pass transmembrane protein that shares 95% amino acid identity with the canine protein. TRAM contains a potential N-terminal N-linked glycosylation site and a C-terminal 60-residue cytosolic portion. Immunoblot analysis showed that TRAM is an abundantly expressed, approximately 36-kD microsomal glycoprotein. Functional analysis indicated that TRAM influences glycosylation and is stimulatory or required for the translocation of secretory proteins.

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Mfr.No.	KTE60137
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Translocating chain-associated membrane protein 1 (TRAM1) ELISA Kit employs a two-site sandwich ELISA to quantitate TRAM1.
Alternative Names	TRAM1; PNAS8; PRO1292; TRAM; TRAMP; translocating chain-associating membrane protein; translocation-associating membrane protein 1
Detection Method	Colorimetric
Kit Components	Human Translocating chain-associated membrane protein 1 microplate Human Translocating chain-associated membrane protein 1 standard Human Translocating chain-associated membrane protein 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Translocating chain-associated membrane protein 1 (TRAM1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TRAM1. No significant cross-reactivity or interference between Human TRAM1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TRAM1 is a multi-pass membrane protein that is part of the mammalian endoplasmic reticulum. The protein influences glycosylation and facilitates the translocation of secretory proteins across the endoplasmic reticulum membrane by regulating which domains of the nascent polypeptide chain are visible to the cytosol during a translocational pause. TRAM is a 374-amino acid, 8-pass transmembrane protein that shares 95% amino acid identity with the canine protein. TRAM contains a potential N-terminal N-linked glycosylation site and a C-terminal 60-residue cytosolic portion. Immunoblot analysis showed that TRAM is an abundantly expressed, approximately 36-kD microsomal glycoprotein. Functional analysis indicated that TRAM influences glycosylation and is stimulatory or required for the translocation of secretory proteins.
Notes	This Human Translocating chain-associated membrane protein 1 (TRAM1) ELISA Kit employs a two-site sandwich ELISA to quantitate TRAM1 in samples. An antibody specific for TRAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRAM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	23471

Applications	ELISA
Applications Notes	This Human Translocating chain-associated membrane protein 1 (TRAM1) ELISA Kit employs a two-site sandwich ELISA to quantitate TRAM1 in samples. An antibody specific for TRAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRAM1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702356	KTE60137	48 T	$339		Add to Cart
BHE13702356	KTE60137	96 T	$589		Add to Cart
BHE13702356	KTE60137	96 Tx5	$2629		Add to Cart
BHE13702356	KTE60137	96 Tx50	$24569		Add to Cart

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