Human transient receptor potential cation channel subfamily V member 4 (TrpV4)ELISA Kit

Human transient receptor potential cation channel subfamily V member 4 (TrpV4)ELISA Kit

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Mfr.No.

CSB-E14199h

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

transient receptor potential cation channel, subfamily V, member 4, OTRPC4, TRP12, VR-OAC, VRL-2, VRL2, VROAC, OSM9-like transient receptor potential channel 4, osmosensitive transient receptor potential channel 4, transient receptor potential channel 12, vanilloid rec

Recovery

The recovery of human CD33 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-104
EDTA plasma (n=4)	95	90-102

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CD33 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-101
1:2	Average %	97
	Range %	91-104
1:4	Average %	98
	Range %	90-113
1:8	Average %	94
	Range %	85-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.130	2.174	2.152	1.993
4000	1.732	1.754	1.743	1.584
2000	1.334	1.355	1.345	1.186
1000	0.826	0.850	0.838	0.679
500	0.565	0.580	0.573	0.414
250	0.420	0.417	0.419	0.260
125	0.302	0.295	0.299	0.140
0	0.154	0.164	0.159	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9HBA0

Applications	ELISA
Research Area	TRPV4

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504652	CSB-E14199h	96T	$790	Add to Cart
BHE10504652	CSB-E14199h	5x96T	$3792	Add to Cart
BHE10504652	CSB-E14199h	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.