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Home / ELISA Kit

Human Transient receptor potential cation channel subfamily V member 1 (TrpV1) ELISA Kit

SKU : BHE13702347

TRPV1 is a nonselective cation channel that may be activated by a wide variety of exogenous and endogenous physical and chemical stimuli. The best-known activators of TRPV1 are heat greater than 43°C and capsaicin, the pungent compound in hot chili peppers.
The activation of TRPV1 leads to painful, burning sensation. Its endogenous activators include: low pH, the endocannabinoid anandamide, N-arachidonoyl-dopamine. TRPV1 receptors are found mainly in the nociceptive neurons of the peripheral nervous system, but they have also been described in many other tissues, including the central nervous system. TRPV1 is involved in the transmission and modulation of pain, as well as the integration of diverse painful stimuli.

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Mfr.No.	KTE60128
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Transient receptor potential cation channel subfamily V member 1 (TrpV1) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPV1.
Alternative Names	TRPV1; DKFZp434K0220; VR1; capsaicin receptor; transient receptor potential vanilloid 1a; transient receptor potential vanilloid 1b; vanilloid receptor subtype 1
Detection Method	Colorimetric
Kit Components	Human Transient receptor potential cation channel subfamily V member 1 microplate Human Transient receptor potential cation channel subfamily V member 1 standard Human Transient receptor potential cation channel subfamily V member 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Transient receptor potential cation channel subfamily V member 1 (TrpV1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TRPV1. No significant cross-reactivity or interference between Human TRPV1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TRPV1 is a nonselective cation channel that may be activated by a wide variety of exogenous and endogenous physical and chemical stimuli. The best-known activators of TRPV1 are heat greater than 43°C and capsaicin, the pungent compound in hot chili peppers. The activation of TRPV1 leads to painful, burning sensation. Its endogenous activators include: low pH, the endocannabinoid anandamide, N-arachidonoyl-dopamine. TRPV1 receptors are found mainly in the nociceptive neurons of the peripheral nervous system, but they have also been described in many other tissues, including the central nervous system. TRPV1 is involved in the transmission and modulation of pain, as well as the integration of diverse painful stimuli.
Notes	This Human Transient receptor potential cation channel subfamily V member 1 (TrpV1) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPV1 in samples. An antibody specific for TRPV1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRPV1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPV1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPV1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	7442

Applications	ELISA
Applications Notes	This Human Transient receptor potential cation channel subfamily V member 1 (TrpV1) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPV1 in samples. An antibody specific for TRPV1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRPV1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPV1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPV1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702347	KTE60128	48 T	$359		Add to Cart
BHE13702347	KTE60128	96 T	$619		Add to Cart
BHE13702347	KTE60128	96 Tx5	$2809		Add to Cart
BHE13702347	KTE60128	96 Tx50	$26209		Add to Cart

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