Human transient receptor potential cation channel subfamily V member 1 (TrpV1)ELISA Kit

Mfr.No.

CSB-E14198h

Alternative Names

transient receptor potential cation channel, subfamily V, member 1, DKFZp434K0220, VR1, capsaicin receptor, transient receptor potential vanilloid 1a, transient receptor potential vanilloid 1b, vanilloid receptor subtype 1

Reactivity

Human

Detection Range

0.312 ng/mL-20 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human CD2AP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	82-94
EDTA plasma (n=4)	93	86-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CD2AP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-93
1:2	Average %	97
	Range %	91-103
1:4	Average %	92
	Range %	87-98
1:8	Average %	101
	Range %	96-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.864	2.755	2.810	2.694
600	2.137	2.212	2.175	2.059
300	1.456	1.537	1.497	1.381
150	0.956	0.946	0.951	0.835
75	0.497	0.489	0.493	0.377
37.5	0.274	0.289	0.282	0.166
18.75	0.218	0.210	0.214	0.098
0	0.116	0.115	0.116	?

Species

Homo sapiens

Sensitivity

0.078 ng/mL

Limit Of Detection

450 nm

UniProt ID	Q8NER1
Antigen	transient receptor potential cation channel, subfamily V, member 1

Applications

ELISA

Research Area

TRPV1

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504650	CSB-E14198h	96T	$790	Add to Cart
BHE10504650	CSB-E14198h	5x96T	$3792	Add to Cart
BHE10504650	CSB-E14198h	10x96T	$7505	Add to Cart

Human transient receptor potential cation channel subfamily V member 1 (TrpV1)ELISA Kit

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