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Home / ELISA Kit

Human Transient receptor potential cation channel subfamily M member 8 (TRPM8) ELISA Kit

SKU : BHE13702348

TRPM8 is an ion channel, upon activation it allows the entry of Na+ (sodium) and Ca2+ (calcium) ions to the cell that leads to depolarization and the generation of an action potential. This eventually leads to the feeling of cold. The TRPM8 protein is expresed in sensory neurons, and it is activated by cold temperatures and cooling agents, such as menthol and icilin where as WS-12 and CPS-369 are the most selective agonist of TRPM8.TRPM8 is also expressed in the prostate, lungs, and bladder where its function is not very well understood.TRPM8 upregulation in bladder tissues correlates with pain in patients with painful bladder syndromes.Furthermore, TRPM8 is upregulated in many prostate cancer cell lines and Dendreon/Genentech are pursuing an agonist approach to induce apoptosis and prostate cancer cell death.

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Mfr.No.	KTE60129
Description	This Human Transient receptor potential cation channel subfamily M member 8 (TRPM8) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPM8.
Alternative Names	TRPM8; LTRPC6; MGC2849; TRPP8; short form of the TRPM8 cationic channel; transient receptor potential subfamily M member 8
Reactivity	Human
Detection Method	Colorimetric
Sample Type	Other biological fluids, Cell culture supernatants, Serum, Plasma
Assay Type	Sandwich (quantitative)
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Components	Human Transient receptor potential cation channel subfamily M member 8 microplate Human Transient receptor potential cation channel subfamily M member 8 standard Human Transient receptor potential cation channel subfamily M member 8 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Transient receptor potential cation channel subfamily M member 8 (TRPM8) ELISA Kit has high sensitivity and excellent specificity for detection of Human TRPM8. No significant cross-reactivity or interference between Human TRPM8 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TRPM8 is an ion channel, upon activation it allows the entry of Na+ (sodium) and Ca2+ (calcium) ions to the cell that leads to depolarization and the generation of an action potential. This eventually leads to the feeling of cold. The TRPM8 protein is expresed in sensory neurons, and it is activated by cold temperatures and cooling agents, such as menthol and icilin where as WS-12 and CPS-369 are the most selective agonist of TRPM8.TRPM8 is also expressed in the prostate, lungs, and bladder where its function is not very well understood.TRPM8 upregulation in bladder tissues correlates with pain in patients with painful bladder syndromes.Furthermore, TRPM8 is upregulated in many prostate cancer cell lines and Dendreon/Genentech are pursuing an agonist approach to induce apoptosis and prostate cancer cell death.
Notes	This Human Transient receptor potential cation channel subfamily M member 8 (TRPM8) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPM8 in samples. An antibody specific for TRPM8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRPM8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPM8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPM8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Gene ID	79054
Antigen	TRPM8
Tag	TRPM8
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Applications	ELISA
Applications Notes	This Human Transient receptor potential cation channel subfamily M member 8 (TRPM8) ELISA Kit employs a two-site sandwich ELISA to quantitate TRPM8 in samples. An antibody specific for TRPM8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRPM8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRPM8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRPM8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702348	KTE60129	48 T	$339		Add to Cart
BHE13702348	KTE60129	96 T	$589		Add to Cart
BHE13702348	KTE60129	96 Tx5	$2629		Add to Cart
BHE13702348	KTE60129	96 Tx50	$24569		Add to Cart

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