Human transglutaminase 2C polypeptide,TGM2 ELISA Kit

Mfr.No.

CSB-E11797h

Sensitivity

4.68 pg/mL

Detection Range

18.75 pg/mL-1200 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase), RP5-1054A22.2, G-ALPHA-h, GNAH, TG2, TGC, C polypeptide, TGase C, TGase-H, protein-glutamine-gamma-glutamyltransferase, tissue transglutaminase, transglutaminase 2, transglutaminase C

Recovery

The recovery of human CFL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	90-96
EDTA plasma (n=4)	105	101-109

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CFL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-93
1:2	Average %	102
	Range %	98-105
1:4	Average %	97
	Range %	95-00
1:8	Average %	88
	Range %	84-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1600	2.604	2.638	2.621	2.486
800	2.078	2.041	2.060	1.925
400	1.329	1.305	1.317	1.182
200	0.804	0.797	0.801	0.666
100	0.431	0.456	0.444	0.309
50	0.294	0.287	0.291	0.156
25	0.213	0.206	0.210	0.075
0	0.136	0.134	0.135	?

Species

Homo sapiens

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P21980

Applications	ELISA
Research Area	TGM2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10504645	CSB-E11797h	96T	$790	Add to Cart
BHE10504645	CSB-E11797h	5x96T	$3792	Add to Cart
BHE10504645	CSB-E11797h	10x96T	$7505	Add to Cart

Human transglutaminase 2C polypeptide,TGM2 ELISA Kit

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