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Home / ELISA Kit

Human Transgelin (TAGLN) ELISA Kit

SKU : BHE13702676

Transgelin (TAGLN) is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of Transgelin is unclear. Two transcript variants encoding the same protein have been found for TAGLN.

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Mfr.No.	KTE60459
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Transgelin (TAGLN) ELISA Kit employs a two-site sandwich ELISA to quantitate TAGLN.
Alternative Names	TAGLN; DKFZp686B01212; DKFZp686P11128; SM22; SMCC; TAGLN1; WS3-10; SM22-alpha; transgelin variant 2
Detection Method	Colorimetric
Kit Components	Human Transgelin microplate Human Transgelin standard Human Transgelin detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Transgelin (TAGLN) ELISA Kit has high sensitivity and excellent specificity for detection of Human TAGLN. No significant cross-reactivity or interference between Human TAGLN and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Transgelin (TAGLN) is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of Transgelin is unclear. Two transcript variants encoding the same protein have been found for TAGLN.
Notes	This Human Transgelin (TAGLN) ELISA Kit employs a two-site sandwich ELISA to quantitate TAGLN in samples. An antibody specific for TAGLN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAGLN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAGLN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAGLN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	6876

Applications	ELISA
Applications Notes	This Human Transgelin (TAGLN) ELISA Kit employs a two-site sandwich ELISA to quantitate TAGLN in samples. An antibody specific for TAGLN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTAGLN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TAGLN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TAGLN bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702676	KTE60459	48 T	$359		Add to Cart
BHE13702676	KTE60459	96 T	$619		Add to Cart
BHE13702676	KTE60459	96 Tx5	$2809		Add to Cart
BHE13702676	KTE60459	96 Tx50	$26209		Add to Cart

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