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Home / ELISA Kit

Human Transformer-2 protein homolog beta (TRA2B) ELISA Kit

SKU : BHE13702360

TRA2-beta gene contains 10 exons and spans 21 kb. RT-PCR and analysis of TRA2-beta cDNAs in the EST database indicated that 5 different TRA2-beta RNA isoforms are generated by alternative splicing.
TRA2-beta-1 is a nuclear protein that colocalizes with SC35 in a speckled pattern. It interacts with several SR proteins in yeast. A second isoform, named TRA2-beta-2, is generated by alternative splicing. This isoform gives rise to a truncated protein without an SR domain. Both isoforms are evenly distributed throughout adult rat tissue. The ratio of these 2 isoforms changes after stimulation of primary human T-cell and primary rat spleen cell cultures, indicating that alternative splicing is involved in the regulation of TRA2-beta activity.

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Mfr.No.	KTE60141
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Transformer-2 protein homolog beta (TRA2B) ELISA Kit employs a two-site sandwich ELISA to quantitate TRA2B.
Alternative Names	TRA2B; DKFZp686F18120; Htra2-beta; SFRS10; SRFS10; TRA2-BETA; TRAN2B; splicing factor; arginine/serine-rich 10; splicing factor; arginine/serine-rich 10 (transformer 2 homolog; Drosophila); transformer 2 hom
Detection Method	Colorimetric
Kit Components	Human Transformer-2 protein homolog beta microplate Human Transformer-2 protein homolog beta standard Human Transformer-2 protein homolog beta detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Transformer-2 protein homolog beta (TRA2B) ELISA Kit has high sensitivity and excellent specificity for detection of Human TRA2B. No significant cross-reactivity or interference between Human TRA2B and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TRA2-beta gene contains 10 exons and spans 21 kb. RT-PCR and analysis of TRA2-beta cDNAs in the EST database indicated that 5 different TRA2-beta RNA isoforms are generated by alternative splicing. TRA2-beta-1 is a nuclear protein that colocalizes with SC35 in a speckled pattern. It interacts with several SR proteins in yeast. A second isoform, named TRA2-beta-2, is generated by alternative splicing. This isoform gives rise to a truncated protein without an SR domain. Both isoforms are evenly distributed throughout adult rat tissue. The ratio of these 2 isoforms changes after stimulation of primary human T-cell and primary rat spleen cell cultures, indicating that alternative splicing is involved in the regulation of TRA2-beta activity.
Notes	This Human Transformer-2 protein homolog beta (TRA2B) ELISA Kit employs a two-site sandwich ELISA to quantitate TRA2B in samples. An antibody specific for TRA2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRA2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRA2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRA2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	615156

Applications	ELISA
Applications Notes	This Human Transformer-2 protein homolog beta (TRA2B) ELISA Kit employs a two-site sandwich ELISA to quantitate TRA2B in samples. An antibody specific for TRA2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTRA2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TRA2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TRA2B bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702360	KTE60141	48 T	$339		Add to Cart
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BHE13702360	KTE60141	96 Tx50	$24569		Add to Cart

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