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Home / ELISA Kit

Human Transcriptional repressor protein YY1 (YY1) ELISA Kit

SKU : BHE13702226

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.
Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins.

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Mfr.No.	KTE60006
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Transcriptional repressor protein YY1 (YY1) ELISA Kit employs a two-site sandwich ELISA to quantitate YY1.
Alternative Names	YY1; DELTA; INO80S; NF-E1; UCRBP; YIN-YANG-1; INO80 complex subunit S; Yin and Yang 1 protein
Detection Method	Colorimetric
Kit Components	Human Transcriptional repressor protein YY1 microplate Human Transcriptional repressor protein YY1 standard Human Transcriptional repressor protein YY1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Transcriptional repressor protein YY1 (YY1) ELISA Kit has high sensitivity and excellent specificity for detection of Human YY1. No significant cross-reactivity or interference between Human YY1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1. Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. May play an important role in development and differentiation. The function of YY1 as an activator or a repressor is specified by the presence of other proteins.
Notes	This Human Transcriptional repressor protein YY1 (YY1) ELISA Kit employs a two-site sandwich ELISA to quantitate YY1 in samples. An antibody specific for YY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YY1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YY1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	7528

Applications	ELISA
Applications Notes	This Human Transcriptional repressor protein YY1 (YY1) ELISA Kit employs a two-site sandwich ELISA to quantitate YY1 in samples. An antibody specific for YY1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyYY1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for YY1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of YY1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702226	KTE60006	48 T	$339		Add to Cart
BHE13702226	KTE60006	96 T	$589		Add to Cart
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BHE13702226	KTE60006	96 Tx50	$24569		Add to Cart

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