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Home / ELISA Kit

Human Transcriptional enhancer factor TEF-1 (TEAD1) ELISA Kit

SKU : BHE13702720

Transcriptional enhancer factor TEF-1 (TEAD1) is a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in TEAD1 cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.

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Mfr.No.	KTE60503
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Transcriptional enhancer factor TEF-1 (TEAD1) ELISA Kit employs a two-site sandwich ELISA to quantitate TEAD1.
Alternative Names	TEAD1; AA; REF1; TCF13; TEF-1; SV40 transcriptional enhancer factor; TEA domain family member 1; atrophia areata; peripapillary chorioretinal degeneration; transcription factor 13; transcriptional enhancer fac
Detection Method	Colorimetric
Kit Components	Human Transcriptional enhancer factor TEF-1 microplate Human Transcriptional enhancer factor TEF-1 standard Human Transcriptional enhancer factor TEF-1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Transcriptional enhancer factor TEF-1 (TEAD1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TEAD1. No significant cross-reactivity or interference between Human TEAD1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Transcriptional enhancer factor TEF-1 (TEAD1) is a ubiquitous transcriptional enhancer factor that is a member of the TEA/ATTS domain family. This protein directs the transactivation of a wide variety of genes and, in placental cells, also acts as a transcriptional repressor. Mutations in TEAD1 cause Sveinsson's chorioretinal atrophy. Additional transcript variants have been described but their full-length natures have not been experimentally verified.
Notes	This Human Transcriptional enhancer factor TEF-1 (TEAD1) ELISA Kit employs a two-site sandwich ELISA to quantitate TEAD1 in samples. An antibody specific for TEAD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTEAD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TEAD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TEAD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	7003

Applications	ELISA
Applications Notes	This Human Transcriptional enhancer factor TEF-1 (TEAD1) ELISA Kit employs a two-site sandwich ELISA to quantitate TEAD1 in samples. An antibody specific for TEAD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTEAD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TEAD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TEAD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13702720	KTE60503	48 T	$339		Add to Cart
BHE13702720	KTE60503	96 T	$589		Add to Cart
BHE13702720	KTE60503	96 Tx5	$2629		Add to Cart
BHE13702720	KTE60503	96 Tx50	$24569		Add to Cart

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