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Home / ELISA Kit

Human Transcription termination factor 1 (TTF1) ELISA Kit

SKU : BHE13702338

TTF2 has been shown to have dsDNA-dependent ATPase activity and RNA polymerase II termination activity. This protein interacts with cell division cycle 5-like, associates with human splicing complexes, and plays a role in pre-mRNA splicing.The deduced 990-amino acid TTF2 protein shares 43% amino acid sequence identity with Drosophila factor 2. These proteins contain 7 motifs found in helicase superfamilies 1 and 2; 7 additional motifs shared only by SWI2/SNF2 family members; and 3 conserved motifs and a nuclear localization signal that are not conserved among the SWI2/SNF2 family. Similar to Drosophila factor 2, recombinant human TTF2 displayed a strong double-stranded DNA-dependent ATPase activity that was inhibited by single-stranded DNA and exhibited RNA polymerase II termination activity.

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Mfr.No.	KTE60119
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Transcription termination factor 1 (TTF1) ELISA Kit employs a two-site sandwich ELISA to quantitate TTF1.
Alternative Names	TTF1
Detection Method	Colorimetric
Kit Components	Human Transcription termination factor 1 microplate Human Transcription termination factor 1 standard Human Transcription termination factor 1 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Transcription termination factor 1 (TTF1) ELISA Kit has high sensitivity and excellent specificity for detection of Human TTF1. No significant cross-reactivity or interference between Human TTF1 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	TTF2 has been shown to have dsDNA-dependent ATPase activity and RNA polymerase II termination activity. This protein interacts with cell division cycle 5-like, associates with human splicing complexes, and plays a role in pre-mRNA splicing.The deduced 990-amino acid TTF2 protein shares 43% amino acid sequence identity with Drosophila factor 2. These proteins contain 7 motifs found in helicase superfamilies 1 and 2; 7 additional motifs shared only by SWI2/SNF2 family members; and 3 conserved motifs and a nuclear localization signal that are not conserved among the SWI2/SNF2 family. Similar to Drosophila factor 2, recombinant human TTF2 displayed a strong double-stranded DNA-dependent ATPase activity that was inhibited by single-stranded DNA and exhibited RNA polymerase II termination activity.
Notes	This Human Transcription termination factor 1 (TTF1) ELISA Kit employs a two-site sandwich ELISA to quantitate TTF1 in samples. An antibody specific for TTF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTTF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TTF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TTF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	7270

Applications	ELISA
Applications Notes	This Human Transcription termination factor 1 (TTF1) ELISA Kit employs a two-site sandwich ELISA to quantitate TTF1 in samples. An antibody specific for TTF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTTF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TTF1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TTF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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