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Home / ELISA Kit

Human Transcobalamin 1 - TCN1 ELISA Kit

SKU : BHE12103921

Binds vitamin B12 with femtomolar affinity and protects it from the acidic environment of the stomach.

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Bioassay Technology Laboratory

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+1 866.986.9598

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Mfr.No.

E2383Hu

Sensitivity

9.58mg/L

Detection Range

20-4500mg/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Haptocorrin;HC;Protein R;TC I;TC1;TC-1;TCI;TCN1;Transcobalamin I;Transcobalamin-1

Molecular Weight

48207

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2383Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	831.23	38.03	4.58
Intra-Assay	2	18	1150.19	40.07	3.48
Intra-Assay	3	18	2036.73	59.92	2.94

Background

Binds vitamin B12 with femtomolar affinity and protects it from the acidic environment of the stomach.

Protocol

These standard curves of E2383Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400mg/L	1.978	1.952	1.91
	1.927
1200mg/L	0.891	0.918	0.876
	0.945
600mg/L	0.412	0.463	0.42
	0.513
300mg/L	0.218	0.193	0.15
	0.167
150mg/L	0.146	0.135	0.093
	0.124
0mg/L	0.045	0.042	0
	0.039

Gene ID	6947
UniProt ID	P20061

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103921	E2383Hu	48T	$320		Add to Cart
BHE12103921	E2383Hu	96T	$458		Add to Cart

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